Hyphenated Techniques B.pharm sem 8 Notes

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Tell me in detail about Hyphenated Techniques

Hyphenated techniques refer to the combined use of two or more analytical techniques to obtain more detailed and accurate information about a sample. These techniques are becoming increasingly popular because they provide more comprehensive data and improve the sensitivity and selectivity of the analysis.


The basic idea behind hyphenated techniques is to use one analytical technique to isolate and concentrate a specific compound or group of compounds, and then use another analytical technique to identify and quantify them. This approach can be useful in a variety of fields, including chemistry, medicine, and environmental science, as it allows researchers to study complex samples more effectively.


Some of the most common hyphenated techniques include:


1. Gas Chromatography-Mass Spectrometry (GC-MS)


GC-MS is one of the most widely used hyphenated techniques in analytical chemistry. It combines gas chromatography, which separates complex mixtures of compounds based on their physical and chemical properties, with mass spectrometry, which measures the mass-to-charge ratio of ionized molecules. This technique is ideal for identifying and quantifying small organic compounds, such as drugs and metabolites.


2. Liquid Chromatography-Mass Spectrometry (LC-MS)


LC-MS is similar to GC-MS, but it uses liquid chromatography instead of gas chromatography. It is commonly used to analyze larger, more complex compounds, such as proteins and amino acids. This technique is also useful for studying biological samples, such as blood and urine.


3. Fourier Transform Infrared Spectroscopy-Mass Spectrometry (FTIR-MS)


FTIR-MS combines the analytical techniques of Fourier Transform Infrared Spectroscopy (FTIR) and Mass Spectrometry (MS). FTIR is used to identify functional groups and chemical bonds in a sample, while MS is used to determine the molecular weight and structure of the compounds present. This technique is often used in the analysis of polymers, plastics, and organic materials.


4. Liquid Chromatography-Nuclear Magnetic Resonance Spectroscopy (LC-NMR)


LC-NMR combines liquid chromatography with nuclear magnetic resonance spectroscopy (NMR). This technique separates and identifies compounds based on their physical and chemical properties, while NMR provides detailed information about the chemical structure and bonding of the compounds. This technique is often used in the analysis of natural products, such as plant extracts and essential oils.


Overall, hyphenated techniques offer a powerful tool for researchers in a variety of fields because they allow for more comprehensive and detailed analysis of complex samples. By combining multiple analytical techniques, researchers can obtain a wealth of information about the compounds present in a sample, leading to a better understanding of how they interact and impact the environment and human health.