Pharmaceutical Biotechnology GTU SEM 5 2019-22 Qustion paper

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Seat No.: _____ Enrolment No. _____________

GUJARAT TECHNOLOGICAL UNIVERSITY
B.Ph. – SEMESTER–V • EXAMINATION – WINTER -2022

Subject Code:BP505TT Date:28/12/2022
Subject Name: Pharmaceutical Biotechnology
Time: 10:30am to 01:30pm Total Marks: 80
Instructions:

1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) Discuss applications of Biotechnology in Pharmaceutical Industry. 06
(b) Write applications of Enzyme Immobilization. Discuss physical entrapment 05

method of Enzyme Immobilization.
(c) What is biosensor? Discuss components of biosensors. 05

Q.2 (a) What is Recombinant DNA technology. Write steps involved in Recombinant 06
DNA Technology.

(b) Define Mutation. Discuss types of mutation. 05
(c) Write a note of Polymerase Chain Reaction. 05

Q.3 (a) Draw a well labled diagram of ideal industrial the fermenter. Explain brief the 06
controls of the Fermenter.

(b) Discuss production of Penicillin with flow chart. 05
(c) Differentiate between Eukeryotes and Prokaryotes. 05

Q.4 (a) Write short note on Hybridoma Technology in production of monoclonal 06
antibody.

(b) Discuss insulin production by rDNA technology. 05
(c) Explain working of ELIZA. 05

Q.5 (a) Explain general method of preparation of bacterial vaccine. 06
(b) Define terms: 1. Bacterial Vaccine 2. Antitoxins 3. Immunoglobulins, 4. 05

Active immunity 5. Passive immunity.
(c) Give structure and function of major hystocompatibility complex. 05

Q. 6 (a) Discuss microbial generic method of conjugation. 06
(b) List out types of hypersensitivity reactions and write on Type-I hypersensitivity 05

reactions.
(c) Discuss applications of recombinant DNA technology. 05

Q.7 (a) Discuss production of enzyme-amylase. 06
(b) Write a note on microbial biotransformation. 05
(c) Write a short note on plasma substitute. 05

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`Seat No.: _____ Enrolment No. _____________

GUJARAT TECHNOLOGICAL UNIVERSITY
B.Pharm. – SEMESTER– V • EXAMINATION – SUMMER -2022

Subject Code: BP505TT Date: 31/05/2022
Subject Name: Pharmaceutical Biotechnology
Time: 02:30pm to 05:30pm Total Marks: 80
Instructions:

1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) Write a note on Enzyme Immobilization and its applications. 06
(b) Give applications of Pharmaceutical Biotechnology 05
(c) Write a note on Polymerase Chain Reactions (PCR) 05

Q.2 (a) Define Immunity. Write a note on Hypersensitivity Reactions 06
(b) Define Mutation. Give types of Mutation. 05
(c) What are antibodies? Write a detail note on Immunoglobulins. 05

Q.3 (a) Draw neat and Clean diagram of Ideal Fermenter. Explain various controls of 06
it.

(b) Explain Production of Vitamin B 12 with flow chart. 05
(c) What are the components of biosensors? 05

Q.4 (a) Explain Production of Penicillins with flow chart. 06
(b) Differentiate Eukaryotes and Prokaryotes 05
(c) Explain Principle and Procedure of Western blotting. 05

Q.5 (a) Explain basic steps of recombinant DNA technology with flow diagram. 06
(b) Differentiate Active immunity and Passive immunity 05
(c) Give the brief application of genetic engineering in medicine. 05

Q. 6 (a) Give the list of different mutagenic agents. How mutants are identify. 06
(b) Write a note on Hybridoma technology 05
(c) Write a note on ELISA 05

Q.7 (a) Write a note on Production of Hepatitis B vaccine. 06
(b) Enlist Blood Products. Write a note on collections, Processing and storage of 05

Whole Human Blood.
(c) Write a note on Monoclonal antibody Production and its applications. 05

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Seat No.: _____ Enrolment No. _____________

GUJARAT TECHNOLOGICAL UNIVERSITY
B.Ph. – SEMESTER–V • EXAMINATION – WINTER -2021

Subject Code:BP505TT Date:22/12/2021
Subject Name: Pharmaceutical Biotechnology
Time:10:30am to 01:30pm Total Marks: 80
Instructions:

1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) Enumerate the methods of recombinant DNA technology. Discuss any one in 06
detail.

(b) Define gene cloning. Discuss gene cloning by Restriction endonuclease. 05
(c) Write detail note on PCR. 05

Q.2 (a) Define Biotechnology and Write detail note on application of Biotechnology in 06
Pharmaceutical field.

(b) Discuss any two methods of enzyme Immobilization. 05
(c) What is Biosensor? Discuss applications of biosensors in Pharmaceutical 05

Industries.

Q.3 (a) Write a note on collection, processing and storage of whole human blood. 06
(b) Differentiate Humoral and Cellular immunity, Active and passive immunity. 05
(c) Write a note on immunoglobulins. 05

Q.4 (a) Write a note Hybridoma technology. 06
(b) Write a note on ELISA. 05
(c) Discuss types of mutations. 05

Q.5 (a) Discuss how the mutants are isolated? 06
(b) Discuss General method of the preparation of bacterial vaccines and toxoids. 05
(c) Discuss General consideration for production of enzyme “Amylase”. 05

Q. 6 (a) Define fermentation. Draw labeled diagram of typical fermenter and give role 06
of different parts of fermenter.

(b) Write short notes on Penicillin’s fermentative production. 05
(c) Write a note on media used for fermentation. 05

Q.7 (a) Write a detail note on Application of r DNA technology and genetic 06
engineering in the production of Hormone- “Insulin”.

(b) Write a note on Hypersensitivity reactions. 05
(c) Differentiate transformation and transduction. 05

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Seat No.: _____ Enrolment No. _____________

GUJARAT TECHNOLOGICAL UNIVERSITY
B.Pharm – SEMESTER– V • EXAMINATION – SUMMER-2021

Subject Code: BP505TT Date: 07/08/2021
Subject Name: Pharmaceutical Biotechnology
Time: 02:30 p.m. – 05:30 p.m. Total Marks: 80
Instructions:

1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) Define enzyme immobilization – write any one method of enzyme 06

immobilization
(b) Write a brief note on biosensors. 05
(c) What in protein engineering. Write on examples of proteins engineered using in 05

silico approaches.

Q.2 (a) Give applications in protein engineering in pharma and medicine. 06
(b) Explain uses of microbes in biotech and pharma industries. What are plasmids 05

and transposones.
(c) Show microbial production of amylase in the form of a flow chart. 05

Q.3 (a) Write on any one 06
1. penicillinase 2. Hybridome technology 3. Proteases

(b) Explain basic steps involved in genetic engineering. 05
(c) Write on application of genetic engineering in pharma and biotech. 05

Q.4 (a) Write in short on any one 06
1. Cloning vectors 2. Restriction endonucleases 3. DNA ligase

(b) write on PCR. 05
(c) How insulin is produced using rDNA technology? 05

Q.5 (a) Classify immunoglobulins draw their basic structure showing differences. 06
(b) Give general functions of immunoglobulins 05
(c) Write on properties of immunoglobulins. 05

Q. 6 (a) Write brief on any one 06
1. Bacterial vaccines 2. Toxoids and anti-toxins 3. Mutagenic agent

(b) Give an overview on hyper sensitivity reactions 05
(c) Explain molecular mechanism of immunosupression, write in brief on 05

immunosuppressive medications.

Q.7 (a) Write in brief on human blood derivatives. 06
(b) Explain working of ELISA or western blotting 05
(c) Write on blood components and their preparation. 05

 

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Seat No.: _____ Enrolment No. _____________

GUJARAT TECHNOLOGICAL UNIVERSITY
B.Pharm. – SEMESTER– V • EXAMINATION – WINTER -2020

Subject Code: BP505TT Date: 13/01/2021
Subject Name: Pharmaceutical Biotechnology
Time: 10:30AM TO 12:30PM Total Marks: 54
Instructions:

1. Attempt any THREE questions from Q-1 to Q-6.
2. Q.7 is compulsory to attempt.
3. Make suitable assumptions wherever necessary.
4. Figures to the right indicate full marks.

Q.1 (a) Write a short note on PCR. 06
(b) Give brief note on production of hepatitis B vaccines. 05
(c) Write a note on production of Enzyme Amylase. 05

Q.2 (a) Explain Structure and Function of MHC. 06
(b) Give working and applications of biosensors. 05
(c) What are antibodies? Write a detail note on Immunoglobulins. 05

Q.3 (a) Give the flow sheet of Vitamin B12 production, isolation and recovery by 06
fermentation.

(b) Write a brief note on PBR 322 vector. 05
(c) Explain general method of the preparation of bacterial vaccines. 05

Q.4 (a) Write a short note on Hypersensitivity reactions. 06
(b) Define enzyme immobilization and explain different techniques adopted. 05
(c) Detail discussion on Recombinant DNA technology. 05

Q.5 (a) Discuss Microbial genetic method of conjugation. 06
(b) Give the difference between humoral immunity vs cellular immunity. 05
(c) Give the brief application of genetic engineering in medicine. 05

Q. 6 (a) Design Fermenter and explain its various controls. 06
(b) Give the list of different mutagenic agents. How mutants are identify. 05
(c) Write a note on Hybridoma technology. 05

Q.7 (a) Give the flow sheet of Griseofulvin production, isolation and recovery by 06
fermentation.

OR
(a) Explain Principle and Procedure of Western blotting. 06

OR
(a) Write a note on whole human blood and its storage. 06

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Seat No.: _____ Enrolment No. _____________

GUJARAT TECHNOLOGICAL UNIVERSITY
B.Ph. – SEMESTER–5 • EXAMINATION – SUMMER -2020

Subject Code: BP505TT Date: 02-11-2020
Subject Name: Pharmaceutical Biotechnology
Time: 2:30 PM TO 5:30 PM Total Marks: 80
Instructions:

1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) Define Biosensors. Give Working and Applications of Biosensors in field of 06
Pharmacy.

(b) Write a note on Protein Engineering. 05
(c) Give applications of Pharmaceutical Biotechnology. 05

Q.2 (a) Write a note on Enzyme Immobilization and its applications. 06

(b) Write a note on Polymerase Chain Reactions (PCR). 05
(c) Define Recombinant DNA Technology and give its applications. 05

Q.3 (a) Write a note on Humulin. 06
(b) Write a note on Production of Hepatitis B vaccine. 05
(c) Explain role of Cloning Vectors, Restriction endonucleases enzymes and DNA 05

ligases enzymes in genetic engineering.

Q.4 (a) Define Immunity. Draw neat and clean structure of Immunoglobulins. 06
(b) Write a note on Hypersensitivity Reactions. 05
(c) Give structure and functions of Major Histocompatibility Complex (MHC). 05

Q.5 (a) Write a note on Monoclonal antibody Production and its applications. 06
(b) Enlist Blood Products. Write a note on collections, Processing and storage of 05

Whole Human Blood.
(c) Give Ideal Properties of Plasma Substituent. Write a note on Dextran. 05

Q. 6 (a) Define Mutation. Give types of Mutation. 06
(b) Write a note on Griffith Theory of Transformation. 05
(c) Write a note on Immuno blotting Techniques. 05

Q.7 (a) Draw neat and Clean diagram of Ideal Fermenter. Explain various controls of 06
it.

(b) Explain Production of Penicillins with flow chart. 05
(c) Explain Production of Vitamin B 12 with flow chart. 05

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Seat No.: ________ Enrolment No.______________

GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM SEMESTER-V- EXAMINATION – WINTER 2019

Subject code: BP505TT Date: 26/11/2019
Subject Name: Pharmaceutical Biotechnology
Time: 02:30 PM TO 05:30 PM Total Marks: 80

Instructions:

1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) Give application of biotechnology in pharmaceutical sciences. 06
(b) Enumerate methods of immobilization and explain entrapment in brief. 05
(c) What are the components of biosensors? 05

Q.2 (a) Describe general consideration of enzyme production with flow chart. 06
(b) Write commercial uses of following industrial enzymes: 05

Amylase, Lipase, Catalase, Protease and Pectinase
(c) Give ideal properties of plasma substitutes. Give the difference between 05

Plasma and Serum

Q.3 (a) Explain basic steps of recombinant DNA technology with flow diagram. 06
(b) What are official blood products and explain anticoagulants. 05
(c) What is PCR? Write its application. 05

Q.4 (a) Explain Immunoglobulin and write its function. 06
(b) Describe salient features of MHC. 05
(c) Differentiate B cell and T cell in immune system. 05

Q.5 (a) Enumerate types of hypersensitivity reaction and explain anaphylactive 06
hypersensitivity.

(b) Differentiate Active immunity and Passive immunity 05
(c) Write application of hybridoma technology. 05

Q. 6 (a) Describe types of biotransformation reactions. 06
(b) Differentiate Eukaryotes and Prokaryotes 05
(c) Explain role of mutation in biotechnology 05

Q. 7 (a) Give ideal requirement of fermenter and sketch the general diagram of it. 06
(b) Enumerate the methods of sterilization and explain moist heat 05

sterilization.
(c) Flow diagram of penicillin production by fermentation 05

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