GUJARAT TECHNOLOGICAL UNIVERSITY
Bachelor of Pharmacy
Subject Code: BP811TT
Page 1 of 2
w.e.f. AY 2017-18
SEMESTER: VIII
Subject Name: Advanced Instrumentation Techniques
Scope: This subject deals with the application of instrumental methods in qualitative and quantitative
analysis of drugs. This subject is designed to impart advanced knowledge on the principles and
instrumentation of spectroscopic and chromatographic hyphenated techniques. This also emphasizes on
theoretical and practical knowledge on modern analytical instruments that are used for drug testing
Objectives
Upon completion of the course the student shall be able to
ysis of drugs.
Teaching scheme and examination scheme:
Teaching Scheme Evaluation Scheme
Theory Tutorial Practical Total Theory Practical
External Internal External Internal
3 1 0 4 80 20 0 0
Sr No Topics %
weightage
1. Nuclear Magnetic Resonance spectroscopy
Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical
shift, coupling constant, Spin – spin coupling, relaxation, instrumentation and
applications
Mass Spectrometry- Principles, Fragmentation, Ionization techniques –
Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of
flight and Quadrupole, instrumentation, applications
10
2. Thermal Methods of Analysis: Principles, instrumentation and applications
of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA),
Differential Scanning Calorimetry (DSC)
X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, Xray
Crystallography, rotating crystal technique, single crystal diffraction,powder
diffraction, structural elucidation and applications.
10
3. Calibration and validation-as per ICH and USFDA guidelines
Calibration of following Instruments
Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer,
Fluorimeter, Flame Photometer, HPLC and GC
10
4.
Radio immune assay:Importance, various components, Principle, different
methods, Limitation and Applications of Radio immuno assay
Extraction techniques:General principle and procedure involved in the solid
phase extraction and liquid-liquid extraction
8
5. Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS. 5
Recommended Books (Latest Editions)
1. Instrumental Methods of Chemical Analysis by B.K Sharma
2. Organic spectroscopy by Y.R Sharma
3. Text book of Pharmaceutical Analysis by Kenneth A. Connors
4. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel