B.Pharm 8 sem GTU syllabus copy Advanced Instrumentation Techniques

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Bachelor of Pharmacy

Subject Code: BP811TT

Page 1 of 2

w.e.f. AY 2017-18


Subject Name: Advanced Instrumentation Techniques

Scope: This subject deals with the application of instrumental methods in qualitative and quantitative

analysis of drugs. This subject is designed to impart advanced knowledge on the principles and

instrumentation of spectroscopic and chromatographic hyphenated techniques. This also emphasizes on

theoretical and practical knowledge on modern analytical instruments that are used for drug testing


Upon completion of the course the student shall be able to

ysis of drugs.

Teaching scheme and examination scheme:

Teaching Scheme Evaluation Scheme

Theory Tutorial Practical Total Theory Practical

External Internal External Internal

3 1 0 4 80 20 0 0

Sr No Topics %


1. Nuclear Magnetic Resonance spectroscopy

Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical

shift, coupling constant, Spin – spin coupling, relaxation, instrumentation and


Mass Spectrometry- Principles, Fragmentation, Ionization techniques –

Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of

flight and Quadrupole, instrumentation, applications


2. Thermal Methods of Analysis: Principles, instrumentation and applications

of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA),

Differential Scanning Calorimetry (DSC)

X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, Xray

Crystallography, rotating crystal technique, single crystal diffraction,powder

diffraction, structural elucidation and applications.


3. Calibration and validation-as per ICH and USFDA guidelines

Calibration of following Instruments

Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer,

Fluorimeter, Flame Photometer, HPLC and GC



Radio immune assay:Importance, various components, Principle, different

methods, Limitation and Applications of Radio immuno assay

Extraction techniques:General principle and procedure involved in the solid

phase extraction and liquid-liquid extraction


5. Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS. 5

Recommended Books (Latest Editions)

1. Instrumental Methods of Chemical Analysis by B.K Sharma

2. Organic spectroscopy by Y.R Sharma

3. Text book of Pharmaceutical Analysis by Kenneth A. Connors

4. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel