Biotechnology sem6 (unit2) hand written notes pdf

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Cloning vectors are artificially created DNA molecules that are used to transfer foreign genetic material into a host organism, such as bacteria or yeast. They contain specific sequences of DNA that allow them to replicate inside cells and carry the foreign DNA along with them.

Cloning vectors come in different types, each with their own specific characteristics and advantages. Some of the most commonly used cloning vectors include plasmids, bacteriophages, cosmids, BACs (bacterial artificial chromosomes), and YACs (yeast artificial chromosomes).

Plasmids are small circular DNA molecules that exist independently within bacteria cells. They can be easily manipulated in the lab and can carry small to medium sized pieces of DNA. Bacteriophages are viruses that can infect and replicate inside bacteria cells. They can carry larger pieces of DNA but are more difficult to work with than plasmids.

Cosmids are hybrid molecules that combine the features of plasmids and bacteriophages. They can carry larger pieces of DNA than plasmids but are more stable than phages. BACs are large DNA molecules that can carry inserts up to 300 kB, while YACs are created for the purpose of carrying large inserts up to several hundred kb.

Cloning vectors contain several important elements that allow them to function properly. These include an origin of replication, which allows the vector to replicate inside cells, a selectable marker, which allows researchers to select cells that have taken up the vector, and a polylinker site or multiple cloning site, which provides a series of restriction enzyme sites for inserting the foreign DNA.

Overall, cloning vectors play an important role in genetic engineering and biotechnology. By using these tools, scientists are able to transfer desired genetic material into host cells, allowing for the replication and expression of the foreign DNA.