1. Morphology of clostridium 
2. spores produced only under anaerobic conditions
3. Clostridium tetani Drum stick (Terminal, spherical)
4. Cl.botulinum Tennis racquet (Terminal, Oval)
5. Cl.chauvoei Citron or Lemon (central, oval)
6. Cl.septicum snow shoe shape ( sub terminal, oval)
7.  Saprophytes found in Soil,
8. Common media –
9.  Blood agar- Haemolytic colony
10. (Double zone haemolytic pattern seen in Cl. perfringen .)
11.  Egg agar,
12.  Cooked meat medium (RCM)
13.  Thioglycolate broth
14. Important species:
15.  Clostridium tetani
16.  Cl.botulinum
17.  Cl.novyi, (odematiens)
18.  Cl.chauvoei
19.  Cl.septicum
20.  Cl.perfringes, (welchii)
21.  Cl.haemolyticum
22. ✓ Toxigenic strain are classified into four groups depending
23. upon the tissue they affect viz,
24. Saccharolytic Proteolytic
25. Organisms ferment
26. carbohydrates of medium
27. Organisms utilize protein of
28. medium
29. Meat particles become pink Meat particle becomes black
30. Gas bubbles are seen Gas bubbles with foul odour
31. 1. Cl.novyi, (odematiens)
32. 2. Cl.chauvoei,
33. 3. Cl.septicum,
34. 4. Cl.perfringes,
35. 1. Clostridium tetani,
36. 2. Cl.botulinum

3. Cl.haemolyticum,