Mycobacteria

➢1882 Robert Koch discovered tubercle bacilli

➢Ehrlich discovered acid fast property of mycobacterium

➢John described johne’s bacilli

CLASSIFICATION OF

MYCOBACTERIA

Mycobacterial (acid-fast) cell wall

CLASSIFICATION OF

MYCOBACTERIA

3. Non Cultivable Mycobacteria

Mycobacteium leprae

Cell wall structure

Morphology

• Acid fast (Z-N positive), rod shape, aerobic, non motile

and non-spore forming.

• Cytochemically : Gram +Ve

• Mycobacteria means “ Fungus bacterium” because some

time mycobacterium show branching filamentus

resembling to fungus mycelium in liquid medium.

Biochemical reaction

Mycobacterium bovis

• Bovine TB can be transmitted from livestock to

humans, deer and other animals

Diagnosis of Bovine TB

• Ziehl Neelsen staining

 Principle: Heat fixed impression smears are stained with

carbol fuchsin, rinsed and counterstained with malachite green.

Interpretation: Acid fast organisms (mycobacteria) retain

their red color and are distinguished from other material and

microorganisms which absorb the counter stain.

• M. bovis isolates – Stone brink’s medium

• M. tuberculosis & M.avium – Lowenstein Jensen slopes

3. Animal inoculation

•M. bovis- Guinea pigs, Rabbits

•M. tuberculosis- Guinea pigs, Rabbits

•M.avium- Chicken,Rabbit

Tuberculin test .

Immuno-diagnosis of tuberculosis

• Leprosy in human

• M.leprae grow on foot pad of mice and Nine

banded armadillo

PARATUBERCULOSIS

➢Johne and Frothingham (1894) first described

Paratuberculosis

➢Acid fast rods , found in clump (Bunch of faggot)

mycobactin killed extract of M. phlehi o

Sheep’s Prolapse of colon in Sheep

Enlarged mesentric lymph node

VACCINE FOR CALF